Gold Nanoparticle Conjugation Optimization Kit - Dot-Blot OPTIONAL:No Validation Kit 64E-10 * All concentrations as

64E-10 * All concentrations as indicated at an optical density of 2

10nm Maleimide-Activated Gold Nanoparticles (lyophilized) - 10 vials

80nm Maleimide-Activated Gold Nanoparticles (lyophilized) - 3 vials

1mM phosphate buffered saline

Absorption max: 553nm

Gold Nanoparticle Conjugation Optimization Kit - Dot-Blot OPTIONAL:No Validation Kit 64E-10 * All concentrations asSupplied with Kit 20nm and 30nm standard gold nanoparticles (100ml each, conc. OD1) 12 conjugation optimization buffers (1. 5ml each) 10% NaCl (100ml) Protein resuspension buffer Detailed conjugation optimization protocol Kit contains enough material to optimize 10 protein conjugates, or one optimization reaction and scale up production of 20ml of conjugate at a final optical density of OD3. Note: Only use with Cytodiagnostics standard or reactant