Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Zinc Size:500g Vegetable Peptone 10 g/L Yeast

Vegetable Peptone 10 g/L Yeast extract 7 g/L Potassium Dihydrogen Phosphate 1

SIH Amino acids drop-out mixtures are used in combination with SIH Minimal medium w/o Amino acids or SIH Minimal medium w/o Amino acids and w/o Ammonium chloride to complete the medium

The medium is used to test the requirement of essential vitamins

Component g/l Peptone 14 Yeast Extract 7 KH2PO4 0

12 pH (100 g/l) 5

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Zinc Size:500g Vegetable Peptone 10 g/L YeastZinc sulphate, ZnSO4. 7H20 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Zinc or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room