Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Boron Size:1000g Final concentration is 40mM Tris

Final concentration is 40mM Tris Base

5 Na2HPO4 0

Yeast extract 5 Dextrose 30 Agar 17 Total 52

By omitting Folic acid and Riboflavine from the original YNB w/o Amino acids formulation the result is a very low fluorescence back ground medium

0 Beef Extract 5

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Boron Size:1000g Final concentration is 40mM TrisBoric acid, HBO3, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Boron or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium Nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room